Table 2. Experimental design and NA sequence results of residue 292.

Day	OC (μg/L)*	G1						G2						G3						G4						G5
	(%RSD)	E1		E2		E3		E1		E2		E3		E1		E2		E3		E1		E2		E3		E1		E2		E3
0	10 (4–7%)
1
2	3 (9–11%)					K
3						K	K
4
5	1 (3–10%)
6
7									K		K	K
8												K			K		K
9	0																	K
10															R			R
11															R		R		K						k
12																						R		R	R
13																				R	R	R						R
14																				R		R		R		R				R	R
15																											R	R		R
16																											R		R
17																														R

The experiment was repeated three times with three H6N2/R292K resistant IAV variants, each consisting of five overlapping generations of Mallards, two birds in each.

*Oseltamivir carboxylate (OC) concentration in the experimental water with range of relative standard deviations (%RSD) for each of the three experiments at respective exposure level (n = 4 at 10 μg/L, n = 3 at 3 μg/L and n = 4 at 1 μg/L). The three experiments had the same OC concentration in the water. Dashed horizontal lines indicate change in OC concentrations.

G1 = generation one, G2 = generation two etc.

E1 = experiment with NA substitutions R292K + D113N. E2 = experiment with NA substitutions R292K + D141N. E3 = experiment with NA substitutions R292K. K displays lysine, R displays arginine, and k displays a mixed proportion of R and K at NA residue 292, as determined by Sanger sequencing of daily fecal samples of each bird. Wild type genotype correlated to unexposed Mallards (P = 0.000002)