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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Mar 30;34(48):5900–5911. doi: 10.1038/onc.2015.31

Figure 8. RBM38 knockout diminishes the effect of PPM1D knockdown on growth suppression.

Figure 8

(A–B) Western blots were prepared with extracts from MCF7 cells uninduced or induced to knock out RBM38 for 4 days, in which PPM1D was knocked down by siPPM1D as indicated. The blots were then probed with antibodies against RBM38, PPM1D, p53, or actin. (C–D) Left panels, representative images of the colony formation assay. Colony formation assay was performed with MCF7 cells uninduced or induced to knock out RBM38 for 4 days, in which PPM1D was knocked down by siPPM1D as indicated. Right panels, quantification of colonies shown in the left panels from three separate experiments. An asterisk indicates a difference with statistical significance (P < 0.05). (E) A model for the role of the RBM38-PPM1D loop in the p53 pathway.