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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Protein Expr Purif. 2015 May 12;114:1–8. doi: 10.1016/j.pep.2015.05.006

Figure 1. Tricine-SDS polyacrylamide gel and immunoblot analysis of the 45% (v/v) ammonium sulfate precipitate fraction of solubilized membrane proteins of H. modesticaldum.

Figure 1

The silver-stained 15% Tricine-SDS polyacrylamide gel and immunoblotting detection of the 8-kDa c-subunit (AptH), shows HF1FO-ATP synthase complex was enriched in the 45% (v/v) saturated ammonium sulfate ((NH4)2SO4) fraction (lanes 2 and 3, respectively). [lane 1, kDa, protein dual color standards (Bio-Rad, USA); lane 2, 45% (v/v) ammonium sulfate precipitate fraction in 15% Tricine-SDS-PAGE; lane 3, 45% (v/v) Western-blot of the ammonium sulfate precipitate fraction using the AptH antibody against the C-subunit of the H. modesticaldum ATP synthase].