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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Protein Expr Purif. 2015 May 12;114:1–8. doi: 10.1016/j.pep.2015.05.006

Figure 6. Native gel electrophoresis analysis and 2D-electrophoresis analysis of the β-DDM-solubilized intact ATP synthase complex of H. modesticaldum.

Figure 6

(A) The intact F1FO complexes and F1 subcomplexes were identified on the gels of blue native electrophoresis (BN-PAGE) (lanes 2 and 3) and high-resolution clear native electrophoresis (hrCN-PAGE) (lane 4 and 5). Lanes 4 and 5 which represent the clear native gel were stained with Coomassie Blue after completion of the electrophoresis run. (B) A hrCN-PAGE gel strip of the H. modesticaldum HF1FO ATP synthase complex was analyzed by 2D electrophoresis, which showed all individual subunits of the H. modesticaldum HF1FO ATP synthase complex. [lane 1, unstained native protein standard (Life Technologies, USA); lane 2, BN-PAGE of the chloroplast CF1FO ATP synthase complex; lane 3, BN-PAGE of the H. modesticaldum HF1FO ATP synthase complex; lane 4, hrCN-PAGE of the chloroplast CF1FO ATP synthase complex; lane 5, hrCN-PAGE of the H. modesticaldum HF1FO ATP synthase complex].