Fig. 6.

A. Analysis of hepatic bile acid composition by mass spectrometry. Dihydroxylated, trihydroxylated and tetrahydroxylated bile acids from liver extract was quantified and normalized to the total detectable bile acids for each animal. Note that WT and NHERF-1^−/− sham mice had similar percentages of dihydroxylated, trihydroxylated and tetrahydroxylated bile acids. After BDL, the percentage of tetrahydroxylated bile acids increased to 14.3±0.74% in NHERF-1^−/− mice, which is significantly higher than the 8.53±2.62% in WT BDL mice (P<0.01). n=5 or 6 in each group. B. Quantification of hepatic Cyp3a11 mRNA levels by real-time PCR. Data are normalized to Gapdh, and the amount of hepatic Cyp3a11 mRNA from wild-type sham mice is set as 1. Values represent the means ± SD of 5 or 6 animals in each group. WT: wild-type. KO: NHERF-1^−/−.