TABLE 1.
NMR distance and dihedral constraints | HSPB1-ACD |
---|---|
Distance constraints | |
Total NOE | 2021 |
Intraresidue | 228 |
Inter-residue | |
sequential (|i-j| = 1) | 635 |
Medium-range (|i-j| < 4) | 279 |
Long-range (|i-j| > 5) | 879 |
Inter-molecular | 266 |
Total dihedral angle restraints | 230 |
φ (TALOS) | 115 |
ψ (TALOS) | 115 |
Residual Dipolar Couplings (RDCs) | |
1H-15N RDCs | 59 |
Structure statistics | |
Violations (mean ± s.d.) | |
Distance constraints (Å) | 0.16±0.21 |
Dihedral angle constraints (°) | 1.07 ± 1.29 |
Average pairwise r.m.s deviation (Å)a | |
Heavy | 1.63 ± 0.56 |
Backbone | 0.79 ± 0.37 |
Average pairwise r.m.s.d. was calculated among ten refined structures.