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. Author manuscript; available in PMC: 2016 Oct 18.
Published in final edited form as: Chem Commun (Camb). 2015 Oct 18;51(81):15051–15054. doi: 10.1039/c5cc06264k

Table 1.

Thermal denaturation temperatures (Tm’s) and thermal advantages (TA’s) for modified DNA duplexes.a

Tm [ΔTm] (°C)
Probe Sequence 5′-Inv: 3′-Inv 5′-Inv: cDNA 3′-Inv: cDNA TA(°C)
1:2 graphic file with name nihms719477t1.jpg 45.0 [+7.5] 55.5 [+18.0] 55.5 [+18.0] 28.5
3:2 graphic file with name nihms719477t2.jpg 31.5 [−6.0] 44.0 [+6.5] 55.5 [+18.0] 30.5
5:2 graphic file with name nihms719477t3.jpg 33.0 [−4.5] 44.5 [+7.0] 55.5 [+18.0] 29.5
1:4 graphic file with name nihms719477t4.jpg 35.0 [−2.5] 55.5 [+18.0] 46.5 [+9.0] 29.5
1:6 graphic file with name nihms719477t5.jpg 28.5 [−9.0] 55.5 [+18.0] 43.5 [+6.0] 33.0
3:4 graphic file with name nihms719477t6.jpg <15.0 44.0 [+6.5] 46.5 [+9.0] >38.0
5:6 graphic file with name nihms719477t7.jpg <15.0 44.5 [+7.0] 43.5 [+6.0] >35.5
7:2 graphic file with name nihms719477t8.jpg <15.0 <15.0 55.5 [+18.0] -
1:8 graphic file with name nihms719477t9.jpg <15.0 55.5 [+18.0] <15.0 -
3:6 graphic file with name nihms719477t10.jpg 28.5 [−9.0] 44.0 [+6.5] 43.5 [+6.0] 21.5
5:4 graphic file with name nihms719477t11.jpg 32.5 [−5.0] 44.5 [+7.0] 46.5 [+9.0] 21.0
a

ΔTm is calculated relative to the corresponding unmodified dsDNA (Tm = 37.5 °C; Thermal denaturation curves were recorded in medium salt phosphate buffer ([Na+] = 110 mM, [Cl−] = 100 mM, pH 7.0 (NaH2PO4/Na2HPO4), [EDTA] = 0.2 mM) and each [ON] = 0.5 μM; see main text for definition of TA.