Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Summary depicting major steps (left to right) of the automated image analysis routine for a single bead. A collection of Gabor annulus filters are applied to the derivative of a raw image via 2-D convolution to locate the centroid of the bead. Once located, the annular ROI method averages the pixel intensity near the bead’s outer radius where signal is greatest (far right, the ROI shown between red and blue circles).
