Fig. 3.

Matrigel invasion assays showing reduced invasion in MDAMB231 cells with PITX2 knockdown. a Top panel shows the cells in the matrigel invasion chamber prior to the clearing of the top chamber. Cells present both at the top chamber and invaded cells in the bottom chamber are visible. Bottom panel shows cells after clearing the top chamber; thus, only those cells invaded to bottom chamber are visible. Different derivatives of cells used in the experiment are indicated. Statistical significance by paired t test in comparison with non-transduced MDAMB231 cells are shown. (MDAMB231- non-transduced parental cells, pLKO vector—transduced with empty vector, Non-targeting—transduced with a non-targeting sequence, B2M—transduced with shRNA of an unrelated gene beta-2 microglobulin, ko- PITX2—transduced with shRNA of PITX2.) b Percentage of cells present in bottom chamber at 24 and 48 h. c Expression levels of PITX2 in the cells used for experiment as determined by qRT–PCR