Table 1.

Relative expression levels of TWIST1, PITX2, and KRT19 in BM specimens from clinical stage II/III breast cancer patient bone marrows collected prior to therapy

Patient #	Site	Histology	ER	PR	Her-2	Recurrence site	*Twist-1	*PITX2	*KRT19
1	R	IDC	neg	neg	pos		NT	1.55	0.42
	L	IDC	neg	neg	pos		0.85	1.24	1.35
2	L	IDC	neg	neg	neg	Lung	0.62	0.30	0.71
	R	IDC	neg	neg	neg	Lung	0.95	0.51	0.33
3	R	IDC	pos	neg	pos		0.92	0.53	7.47
	L	IDC	pos	neg	pos		0.52	0.73	5.59
4	L	IDC	neg	pos	neg	Lung	3.08	0.94	2.65
	R	IDC	neg	pos	neg	Lung	0.23	3.35	0.34
5	L	IDC	neg	neg	neg	Liver	9.05	42.19	0.57
	R	IDC	neg	neg	neg	Liver	27.86	91.71	1.75
6	X	IDC	neg	neg	neg		0.93	1.52	0.23
	X	IDC	neg	neg	neg		0.47	1.65	3.66
7	R	IDC	neg	neg	neg	Liver	ND	1.25	0.53
	L	IDC	neg	neg	neg	Liver	0.41	0.89	3.98
8	R	IDC	pos	pos	neg		ND	3.47	0.76
9	R	IDC	pos	neg	neg	Liver, bone	1.16	13.99	7.85
	L	IDC	pos	neg	neg	Liver, bone	1.91	23.75	0.23
10	R	IDC	neg	neg	neg		0.67	0.71	3.05
	L	IDC	neg	neg	neg		1.33	5.82	0.56
11	R	IDC	neg	neg	neg		ND	ND	0.40
	L	IDC	neg	neg	neg		0.34	0.98	1.11
12	L	IDC	neg	neg	pos		0.47	1.93	0.93
	R	IDC	neg	neg	pos		0.51	0.51	0.19
13	L	IDC	pos	pos	neg		ND	ND	ND
	R	IDC	pos	pos	neg		0.79	2.23	0.60
14	L	IDC	neg	neg	pos	Brain	0.68	2.28	0.68
	R	IDC	neg	neg	pos	Brain	0.61	16.71	0.47
15	L	IDC	neg	neg	neg		0.83	1.42	5.82
	R	IDC	neg	neg	neg		0.56	1.40	0.12
16	L	ILC	neg	neg	neg	Liver, pancreas	0.42	6.24	3.28
17	X	IDC	pos	pos	neg		0.96	0.41	1.09
18	R	IDC	pos	neg	neg	Liver, lung, bone	0.37	0.24	0.17
	L	IDC	pos	neg	neg	Liver, lung, bone	1.49	0.30	3.25
19	X	IMC	neg	neg	neg		0.32	0.42	0.87
20	L	ILC	neg	neg	neg	Liver, bone	0.78	0.68	0.10
	R	ILC	neg	neg	neg	Liver, bone	1.20	0.91	0.44
21	R	IDC	neg	neg	pos	Contralateral axilla	ND	0.75	0.55
	L	IDC	neg	neg	pos	Contralateral axilla	0.32	0.36	0.54
22	L	IDC	neg	neg	neg		0.91	0.69	0.17
	R	IDC	neg	neg	neg		0.62	1.77	0.35
23	L	IMC	pos	pos	neg	Brain, liver	1.16	1.70	0.64
	R	IMC	pos	pos	neg	Brain, liver	ND	2.08	0.12
24	X	ILC	pos	neg	neg		1.10	1.16	0.96
25	R	ILC	neg	pos	pos	Liver	1.30	0.92	1.12
26	R	IDC	neg	pos	neg		ND	2.55	0.52
27	X	IDC	pos	pos	neg		0.21	3.13	0.33
28	L	IMC	pos	pos	neg		0.19	0.53	3.39
	R	IMC	pos	pos	neg		0.47	3.60	2.01
29	R	IDC	neg	neg	pos	Liver, lung	1.63	0.56	6.27
30	X	IDC	neg	neg	neg		0.34	0.90	3.29

Site- site of BM collection, right or left iliac crest, Histology- IDC, invasive ductal carcinoma; ILC, invasive lobular carcinoma; IMC, invasive mammary carcinoma. PCR values are expressed as fold change in gene expression relative to normal human BM