Table 2.
Effect of dexamethasone (DEX) and mechanical stress on cGMP distribution in cultured rat podocytes
| NS—non-stressed cells | S—stressed cells | ||||
|---|---|---|---|---|---|
| Control | +DEX | Control | +DEX | ||
| nmol cGMP/105 cells/15 min | |||||
| ANP | [cGMP]t | 89 ± 10 | 44 ± 5# | 70 ± 7* | 51 ± 6# |
| [cGMP]e | 16 ± 3 | 14 ± 3 | 39 ± 6* | 30 ± 1 | |
| [cGMP]e (%) [cGMP]t |
18 ± 3 | 38 ± 5# | 52 ± 4* | 61 ± 6* | |
| ANP+Ang II | [cGMP]t | 66 ± 3† | 57 ± 3 | 49 ± 7† | 26 ± 5#*$ |
| [cGMP]e | 14 ± 2 | 19 ± 2 | 34 ± 3* | 15 ± 2# | |
| [cGMP]e (%) [cGMP]t |
21 ± 3 | 28 ± 4 | 72 ± 5†* | 56 ± 2 | |
| SNAP | [cGMP]t | 20 ± 3 | 31 ± 2# | 11 ± 2* | 9 ± 2* |
| [cGMP]e | 8 ± 1 | 14 ± 7 | 9 ± 2 | 7 ± 1* | |
| [cGMP]e (%) [cGMP]t |
34 ± 2 | 36 ± 7 | 78 ± 5* | 81 ± 4*$ | |
The podocytes cultured in 6-well plates with flexible bottom were mechanically stressed for 24 h with or without (control) 1 μM DEX. ANP, SNAP, and Ang II were added for 15 min as described in Materials and Methods. [cGMP]e—extracellular cGMP, [cGMP]t—total cGMP. Results are presented as means from 3 to 4 independent experiments ±SEM
* P < 0.05 versus respective non-stretched cells
# P < 0.05 versus respective control cells
† P < 0.05 versus respective cells without Ang II
$ P < 0.05 versus respective (ANP + DEX) cells