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. 2015 Jul 14;409(1-2):33–43. doi: 10.1007/s11010-015-2509-9

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Cucurbitacin D induces G2/M cell cycle arrest and apoptosis in MCF7/ADR cells. MCF7/ADR cells were treated with cucurbitacin D (0.5 μg/mL) in the presence and absence of doxorubicin (1 μM) for 24 h. Cell cycle distribution was analyzed using a FACS flow cytometer (a). MCF7/ADR cells were treated with the indicated drugs for 24 h and subjected to Annexin V/PI assay. b Effect of cucurbitacin D on the expression of cleaved caspase-3, cleaved caspase-8, and cleaved PARP. MCF7/ADR cells were treated with cucurbitacin D (0.5 μg/mL) in the presence and absence of doxorubicin (1 μM) for 24 h. The cell lysates were subjected to Western blot analysis using specific antibodies (c)

Fig. 6 — Cucurbitacin D induces G2/M cell cycle arrest and apoptosis in MCF7/ADR cells. MCF7/ADR cells were treated with cucurbitacin D (0.5 μg/mL) in the presence and absence of doxorubicin (1 μM) for 24 h. Cell cycle distribution was analyzed using a FACS flow cytometer (a). MCF7/ADR cells were treated with the indicated drugs for 24 h and subjected to Annexin V/PI assay. b Effect of cucurbitacin D on the expression of cleaved caspase-3, cleaved caspase-8, and cleaved PARP. MCF7/ADR cells were treated with cucurbitacin D (0.5 μg/mL) in the presence and absence of doxorubicin (1 μM) for 24 h. The cell lysates were subjected to Western blot analysis using specific antibodies (c)