Fig. 1.

Central GLP-1R activation decreases body weight and food intake and induces activation of BAT in lean mice. Mice were treated for 5 days with i.c.v. exendin-4 (n = 8) or vehicle (control n = 9, pair-fed n = 6). On a daily basis, body weight (a, b) and food intake (c) were monitored. Samples of BAT and WAT were collected and stained for TH and UCP-1. Representative pictures and quantification of TH (d, e), UCP-1 (f, g) and lipid droplet content with H&E staining (h, i) are shown. Values are mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with control; ^† p < 0.01 compared with pair-fed control. White bars, control; black bars, exendin-4; grey bars, pair-fed control