FIG 10.

Model of the regulation of the G₂-to-M transition by cPLA₂α. In wild-type (WT) cells, cPLA₂α directly binds to SIRT2 and promotes phosphorylation of SIRT2 at S331 by cyclin A-Cdk2 complex. This phosphorylation inhibits SIRT2 catalytic activity and decreases enrichment of SIRT2 at the mitotic spindles and centrosomes. In the absence of cPLA₂α, nonphosphorylated SIRT2 is localized to the mitotic structures and arrests cell cycle progression at the G₂-to-M transition under mitotic stress.