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. 2015 Sep 30;35(21):3714–3725. doi: 10.1128/MCB.00298-15

FIG 5.

FIG 5

Deletion of Set3C subunits suppressed DNA damage sensitivity of esa1. (A) Subunit composition of Sum1C and Set3C (with data from reference 39). The relative size of the subunits is drawn to scale. The small green circle in Set3C represents the Cpr1 subunit. (B) Deletion of each Set3C subunit tested suppressed esa1 sensitivity to CPT. Wild-type (LPY6497), esa1-531 (LPY14757), esa1-531 hos2Δ (LPY14761), esa1-531 hos4Δ (LPY15865), esa1-531 set3Δ (LPY15869), esa1-531 snt1Δ (LPY15867), esa1-531 sif2Δ (LPY15863), and esa1-531 hst1Δ2 (LPY18266) strains were tested. (C) Suppression of esa1 DNA damage sensitivity was specific to Set3C. Deletion of SUM1 had no effect on esa1 sensitivity to CPT. Loss of HST1 was comparable to hos2Δ for suppression of esa1 DNA damage sensitivity. Wild-type (LPY6497), esa1-531 (LPY14757), esa1-531 hos2Δ (LPY14761), esa1-531 hst1Δ2 (LPY18266), and esa1-531 sum1Δ (LPY17505) strains were compared. Figure S3 in the supplemental material includes single mutant controls for Set3C subunits.