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. 2015 Sep 30;35(21):3726–3738. doi: 10.1128/MCB.00382-15

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FIG 5 — Effects of yolk sac (YS)-specific ablation of Alk3 or Smad4 on selected targets. EKLF (left), Gata1 (middle), and DEK (right) transcript levels were monitored in yolk sac cells derived from Flk1-Cre (Flk1^+/Cre) × Smad4 (wild-type or fl/fl) E9.5 embryos (bottom) or Flk1-Cre (Flk1^+/Cre) × Alk3 (wild-type or fl/fl) E10.5 embryos (top). Samples were processed for RNA isolation and analyzed by quantitative RT-PCR. Levels are normalized to glyceraldehyde-3-phosphate dehydrogenase and then set to “1” for wild type. Results are averages from two to three independent biological replicates performed each in triplicate.