FIG 3.

Multiple developmental-stage-specific binding activities are observed within the dHS1 region of Fab-7. (A) Gel shift analysis with probes derived from dHS1 of Fab-7 demonstrates stage-specific binding activities. EMSAs were carried out as described in Materials and Methods with ³²P-labeled DNA probes. Probes pdHS1A, G3+4, and G5+6 were incubated with nuclear extracts derived from early (E; 0- to 6-h) and late (L; 6- to 18-h) embryos. The identities of the binding activities with early- and late-stage specificity are represented with blue and black arrowheads, respectively. F, free probe. (B) EMSAs with late nuclear extracts were performed with probes G3+4 (lanes 1 to 10) and G5+6 (lanes 11 to 20) in the absence (lanes 1, 2, 11, and 12) or presence (lanes 3 to 10 and 13 to 20) of cold competitor, as indicated above the lanes. A 50- or 100-fold excess of cold competitor was added to the reaction mixes.