Skip to main content
. 2015 Sep 30;35(21):3657–3668. doi: 10.1128/MCB.00233-15

FIG 1.

FIG 1

Cdc14B−/− MEFs are defective in DNA damage repair at late passages. (A and B) Immunofluorescence (IF) staining of the DNA damage markers γH2AX (A) and 53BP1 (B) in early-passage (P3) and late-passage (P7) MEFs after 10 Gy of IR. (C) Quantification of γH2AX-positive cells (>20 foci) in the P3 and P7 MEFs from panel A. (D) Quantification of 53BP1-positive cells (>10 foci) in the P3 and P7 MEFs from panel B. At least 100 cells were counted for each experiment. The error bars indicate standard deviations for three independent experiments. (E) Neutral comet assay in P3 and P7 MEFs after 10 Gy of IR. (F) Quantification of damaged cells with DNA double-strand breaks from panel E. Tail moments (TMs) were gauged for at least 50 cells in each experiment. The cells with TMs of >5 were counted as damaged cells. The error bars indicate standard deviations for three independent experiments. Statistical significance was assessed by ANOVA (*, P < 0.05; **, P < 0.01).