TABLE 1.
Synthesized oligonucleotides for mutant RSS substratesa
| Substrate | 12RSS region or 12RSS mutant oligonucleotide sequence |
|---|---|
| p12/23 | 5′-ATCTCGCGGCCGCACGCGTTCGAAGTCGAGGTTTTTGTTCCAGTCTGTAGCACTGTGCAGACGTTCCTATTATTTTTGACACCAGACAAGTTGGTAATGGTAGCGACCGGCGCTCAGTTGGAATTCTACG-3′ |
| pScrH/23 | 5′-ATCTCGCGGCCGCACGCGTTCGAAGTCGAGGTTTTTGTTCCAGTCTGTAGCGCTGCGCAGACGTTCCTATTATTTTTGACACCAGACAAGTTGGTAATGGTAGCGACCGGCGCTCAGTTGGAATTCTACG-3′ |
| pScrN/23 | 5′-ATCTCGCGGCCGCACGCGTTCGAAGTCGATCCGACTTATCCAGTCTGTAGCACTGTGCAGACGTTCCTATTATTTTTGACACCAGACAAGTTGGTAATGGTAGCGACCGGCGCTCAGTTGGAATTCTACG-3′ |
| p(CA)38/23 | 5′-ATCTCGCGGCCGCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACGTTGGTAATGGTAGCGACCGGCGCTCAGTTGGAATTCTACG-3′ |
a
The synthesized oligonucleotides (Blue Heron Bio) for the 12RSS sites of pScrH/23, pScrN/23, p(CA)38/23 are listed and compared to the sequence of the 12RSS region of p12/23. The nonamer and heptamer sequences are highlighted in boldface type, while the regions of mutagenesis are underlined.