TABLE 2.
Oligonucleotide primers and probes used for detection of the RAG binding sequencea
| Region | Sequence |
||
|---|---|---|---|
| Forward primer | Hydrolysis probe | Reverse primer | |
| Jκ1 | 5′-TTGTACAGCCAGACAGTGGAG-3′ | 5′-TGGTGCCTCCACCGAACGTC-3′ | 5′-GCCACAGACATAGACAACGG-3′ |
| Jκ2 | 5′-CAGATTCTGGCACTCTCCAA-3′ | 5′-CAAAGAAGCAGGGTAGCCTGCCC-3′ | 5′-ACTGAGCATGGTCTGAGCAC-3′ |
| Jκ5 | 5′-CATAGTCCTCACTGTGGCTCA-3′ | 5′-CTTGGTCCCAGCACCGAACG-3′ | 5′-AGTGTACTTACGTTTCAGCTCCA-3′ |
| Pou2af1 | 5′-ATCTCCGATTCTGTGCATGA-3′ | 5′-AATCTGCCCTTCTGTCACCTCAGTGA-3′ | 5′-GTGAAGAACGGCTCCTAACC-3′ |
| γ-Actin | 5′-CCTCCTCCAATAAAGGGACA-3′ | 5′-AGAACTGGTGACCCTCTCCCACG-3′ | 5′-GCCATCACATCCCAGTCA-3′ |
| α-Actin | 5′-GGCACGTGACACTCTTGTCT-3′ | 5′-ATCGGTGGCTCCATCCTGGC-3′ | 5′-GTCGTACTCCTGCTTGGTGA-3′ |
| 12a | 5′-CCTCTAGACTGCCGGATCTC-3′ | 5′-TCCCTCCTCCAAGCCCTGGA-3′ | 5′-CATAAGCCTGCTGGTTCAGA-3′ |
| 23d | 5′-GCAAGACTGAGAGCAGGAGA-3′ | 5′-CCGTCCTCGAGGCACCATGA-3′ | 5′-ACACCGGCCTTATTCCAA-3′ |
a
Hydrolysis probes were labeled at the 5′ end with the reporter dye 6-carboxyfluorescein (FAM) and at the 3′ end with black hole quencher (BHQ) dye (Biosearch Technologies).