Table 2.
Primers used in this work.
Primer group and name | Sequence (5′ to 3′) |
---|---|
Oligonucleotides for strain construction | |
F-leu3 | TGCAATTATGGAAGGAAGATCAGATTTTGTGGCGACTTCACACAGCTGAAGCTTCGTACGC |
R-leu3 | GGACTTTAAACCTTGGGATTGAACGCAAATTCATTCATTAAACATAGGCCACTAAGTGGATCTG |
F-STP1-HA | CAATATTTGAATTTTTACAATGACAACTTTGGGTCACAATTTCGGATCCCCGGGTTAATTAA |
R-STP1-HA | TTCCAATATGATACCCTTATTTTTATCCCGTGTTATATTTAAGAATTCGAGCTCGTTTAAAC |
F-LEU3 prom | AGGTGCCGCCTAATTTATCG |
R-LEU3 int | ACTTCTGCTGACGACATTCC |
F-KanMX6 int | CATCCTATGGAACTGCCTCG |
R-KanMX6 int | GATAGATTGTCGCACCTGATTG |
F-STP1 int | GCACAAGATAATCCTTCGTTCC |
R-STP1 down | TCGGCTTTCCAATATGATACCC |
R-Kan int | CTATACCTGAGAAAGCAACCTG |
| |
Oligonucleotides for RT-qPCR | |
F-qRT-UGA4 | CTGCTGCTGTCACATTAACC |
R-qRT-UGA4 | AATACACATAACCACCACTGC |
F-qRT-DAL7 | AACCGAACAAATCAGGAAC |
R-qRT-DAL7 | CAAGTTGGAGATGAAGAGTC |
F-qRT-BAP2 | TAGAGGATGGCGTTGAGTC |
R-qRT-BAP2 | ACCAAGATGTAACCAATTATTAGC |
F-qRT-AGP1 | ATCTTATTCCTATTCTTGGCTACC |
R-qRT-AGP1 | CGGCGTTAATGAAGTGTGG |
F-qRT-TBP1 | TATAACCCCAAGCGTTTTGC |
R-qRT-TBP1 | GCCAGCTTTGAGTCATCCTC |
| |
Oligonucleotides for ChIP | |
F-UGA4 | GGAACTGATTACTGTGCCAAG |
R-UGA4 | AATCGCTTATCGCTTATCGTG |
F-AGP1 | TTATACCTCGGCGGCTTC |
R-AGP1 | GCAAGATTTCTCCAAAGTCC |
F-BAP2 | AGGAGGCTACTGACACTGC |
R-BAP2 | GCTGACATATTTACCGTTGAAGG |
F-DAL7 | AATCTCCGCTGAAGTTGC |
R-DAL7 | TTTCACGATGTACCTTATCCAAGA |
F-UC | AGTCCAATACCTCTGTCCTC |
R-UC | AGCCGCAACTTCATTCTG |