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. 2015 Oct 1;6:818. doi: 10.3389/fpls.2015.00818

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Copyright © 2015 Li, Leopold, Sander, Shanks, Zhao and Gibson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course analyses of transcript and metabolite levels from the terpenoid pathway. Results depicted are the following: un-induced CrBPF1-OE line (, solid line), β-estradiol induced CrBPF1-OE line (, solid line), un-induced control line (, dashed line) and β-estradiol induced control line (, dashed line). Relative transcript levels are presented as ΔΔCt. Results shown are the following: (A) DXS1 mRNA levels, (B) DXS2A mRNA levels, (C) DXS2B mRNA levels, (D) G10H mRNA levels (E) CPR mRNA levels, (F) LAMT mRNA levels, (G) loganin levels, and (H) secologanin levels. Results for transcript levels are the average ΔΔCt values of three biological replicates with two technical replicates per biological replicate. Results for metabolite levels are the averages of three biological replicates. Metabolite levels for the control line are not depicted. Error bars indicate SD. Transcript levels in the un-induced versus induced CrBPF1-OE cultures differed at the same time point with: ^∗p ≤ 0.05, ^∗∗p ≤ 0.01 according to a Student’s t-test. The results of Student’s t-tests for the un-induced versus induced cultures of the control line are not depicted. Metabolite levels in the un-induced versus induced CrBPF1-OE cultures differed at the same time point with: ^∗p ≤ 0.1, ^∗∗p ≤ 0.05 according to a Student’s t-test.

Inline graphic — Time course analyses of transcript and metabolite levels from the terpenoid pathway. Results depicted are the following: un-induced CrBPF1-OE line (, solid line), β-estradiol induced CrBPF1-OE line (, solid line), un-induced control line (, dashed line) and β-estradiol induced control line (, dashed line). Relative transcript levels are presented as ΔΔCt. Results shown are the following: (A) DXS1 mRNA levels, (B) DXS2A mRNA levels, (C) DXS2B mRNA levels, (D) G10H mRNA levels (E) CPR mRNA levels, (F) LAMT mRNA levels, (G) loganin levels, and (H) secologanin levels. Results for transcript levels are the average ΔΔCt values of three biological replicates with two technical replicates per biological replicate. Results for metabolite levels are the averages of three biological replicates. Metabolite levels for the control line are not depicted. Error bars indicate SD. Transcript levels in the un-induced versus induced CrBPF1-OE cultures differed at the same time point with: ^∗p ≤ 0.05, ^∗∗p ≤ 0.01 according to a Student’s t-test. The results of Student’s t-tests for the un-induced versus induced cultures of the control line are not depicted. Metabolite levels in the un-induced versus induced CrBPF1-OE cultures differed at the same time point with: ^∗p ≤ 0.1, ^∗∗p ≤ 0.05 according to a Student’s t-test.