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Localization of OsCYP21-4-GFP in N. benthamiana. (A) OsCYP21-4-GFP did not co-localize with the mitochondrial marker, MitoTracker. (B) OsCYP21-4-GFP co-localized with the Golgi Apparatus marker α-ManI-mCherry. (C,D) Subcellular localizations of OsCYP21-4TM-GFP and OsCYP21-4ΔTM-GFP proteins. (E) Co-localization of OsCYP21-4-GFP with the ER marker, BiP-RFP under treatment with or without 50 μM BFA for 3 h. Bars = 20 μm.
