Skip to main content
. 2015 Oct 1;6:797. doi: 10.3389/fpls.2015.00797

Figure 4.

Figure 4

Recombinant OsCYP21-4 lacks PPIase activity in vitro. (A) Recombinant OsCYP21-4 expression was induced with IPTG in E. coli and purified on nickel-NTA agarose columns. Samples were separated by 12% SDS-PAGE and stained with Coomassie blue. Un/In: Total protein un-induced/induced with isopropyl β-D-1-thiogalactopyranoside in E. coli; Pu: Purified OsCYP21-4 protein (star). (B) Traces showing isomerization of the Suc-AAPF-pNA substrate in the absence of OsCYP21-4 (Blank; …) and in the presence of 50 nM (50 nM OsCYP21-4;– -), 100 nM (100 nM OsCTP21-4;– -), and 200 nM (200 nM OsCTY21-4; _ _) recombinant OsCYP21-4 protein. CypD (200 nM CypD;) as a positive control. A representative recording from one of three independent experiments is shown.