Figure 1.

BACE1 overexpression alters APP processing and glucose oxidation in SH-SY5Y cells. (A) Representative immunoblots showing stable overexpression of BACE1 and resultant changes in cellular APP and sAPPα and sAPPβ shed into the culture media, compared to empty vector (EV) treated controls. (B–E) SH-SY5Y BACE1 overexpression did not change APP protein levels (B; n = 4), increased total BACE1 protein levels (C; n = 7) and induced a shift in APP processing, with a reduction in sAPPα (D; n = 4) and an increase in sAPPβ (E; n = 4). (F–H) SH-SY5Y BACE1 overexpression reduced ¹⁴C-glucose oxidation rate (F; n = 7), with no reduction in ¹⁴C-glucose incorporation rate (G; n = 9), giving an overall reduction in the ratio of ¹⁴C-glucose oxidation to incorporation (H; n = 7). Values are means ± SEM. ^**p < 0.01; ^***p < 0.001.