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. 2015 Oct 1;9:382. doi: 10.3389/fncel.2015.00382

Figure 6.

Figure 6

Reversal of BACE1 mediated impaired glucose oxidation in SH-SY5Y cells. (A,B) Representative immunoblots (A) of phosphorylated PDHe1α (p-PDHe1α) subunit, total PDHe1α and actin loading control in control (EV) and BACE1 overexpressing cells in the absence and presence of 10 μM or 100 μM dichloroacetate (DCA), with quantification of the immunoblot data shown in (B) (n = 4). (C) DCA partially reverses BACE1 mediated impairment of glucose oxidation, but also increases OCR of EV cells (n = 5). (D) reduced OCR associated with BACE1 cells is recovered to EV control levels by addition of 1 μM and 10 μM beta hydroxybutyrate (n = 4–6). (E) Representative immunoblots of BCL-2-associated agonist of cell death (BAD) and actin loading control with quantification of the immunoblot data (n = 4). (F) BACE1-mediated reduction in OCR is recovered to EV control levels by addition of 50 μM α-lipoic acid (n = 6). Values are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.