Figure 1.

Growth, expression, and specific styrene epoxidation activity profiles of P. taiwanensis VLB120 (pA-EGFP_B) (A) and P. taiwanensis VLB120 (pStyAB) (B) grown on citrate as sole carbon source. Cells were cultivated in M9* medium and induced with 0.025% (v/v) DCPK after 3 h of growth. Samples for resting-cell activity, specific fluorescence, and SDS-Page analyses were taken at regular time intervals (every 2 h) after induction. For determination of the specific styrene epoxidation activity, cells were harvested, resuspended in KPI buffer containing 0.5% (v/v) citrate, and supplied with 1.5 mM styrene to start the reaction. Growth rates [h⁻¹] were determined during exponential phase (dashed line). Yields on citrate were determined after inoculation and at the time point of citrate depletion. Error bars represent standard deviations of two independent assays.