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. 2015 Oct 1;6:1042. doi: 10.3389/fmicb.2015.01042

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Copyright © 2015 Lindmeyer, Jahn, Vorpahl, Müller, Schmid and Bühler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of growth behavior (A,B) and variability in StyA-eGFP levels (C,D) upon expression under the control of the alk- (A,C) and the lac- (B,D) regulatory systems in P. putida DOT-T1E. Results for 4 representative cultures are shown each for P. putida DOT-T1E (pA-EGFP_B) and P. putida DOT-T1E (pA-EGFP_B_lac) (see text for details on clone selection). Cells were cultivated in M9^* medium supplement with 0.5% (w/v) citrate. Induction was initiated at 0 h by the addition of 0.025% (v/v) DCPK or 1 mM IPTG for the alk- or the lac-based expression, respectively. The specific fluorescence was determined via eGFP fluorescence measurement and normalization to the cell concentration. Error bars give the standard deviations of two independent fluorescence measurements.