Table 1.
Bacterial strains and plasmids used.
| Strain | Characteristics | References |
|---|---|---|
| E. coli DH5α | supE44 ΔlacU169 (Φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Hanahan, 1983 |
| E. coli JM101 | supE thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZ ΔM15] | Messing, 1979 |
| P. taiwanensis VLB120 | Wild-type, pSTY, Tolr | Köhler et al., 2013 |
| P. putida DOT-T1E | Wild-type, pGRT1, Tolr, Rifr | Ramos et al., 1995 |
| P. putida KT2440 | mt-2 pWW0 cured, Tols | Bagdasarian et al., 1981 |
| Plasmid | Characteristics | References |
| pStyAB | pCom10, alk-regulatory system (alkS/PalkB), styAB, Kmr, | Lindmeyer et al., 2015 |
| pStyAB_lac | pCom10-derived, lac-regulatory system (lacI, PlacUV5), styAB, Kmr | Lindmeyer et al., 2015 |
| pStyAB_tac | pCom10-derived, tac-regulatory system (lacIq, Ptac), styAB, Kmr | This study |
| pA-EGFP_B | pCom10, alk-regulatory system (alkS/PalkB), styA-eGFP and styB, Kmr | Jahn et al., 2014 |
| pA-EGFP_B _lac | pCom10-derived, lac-regulatory system (lacI, PlacUV5), styA-eGFP and styB, Kmr | This study |
| pA-EGFP_B _tac | pCom10-derived, tac-regulatory system (lacIq, Ptac), styA-eGFP and styB, Kmr | This study |
| pLac_alaD_ω-TA | Source of (lacI, PlacUV5) | Unpublished |
| pTAC:LS:AGPPS2 | Source of (lacIq, Ptac) | Willrodt et al., 2014 |
Kmr, kanamycin resistance; Tolr, toluene resistance; Tols, toluene sensitive; Rifr, rifampicin resistance.