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Temperature and pH optima of purified OYERo2 (
) and purified OYERo2a (
). (A) Normalized activity-temperature plot: 140 μM NADPH and 1 mM maleimide in 50 mM phosphate buffer (pH 7.1) were incubated at various temperatures (10-50°C) for 10 min. The reaction was started through the addition of 30 nM enzyme (not tempered). (B) Normalized activity-pH plot: 140 μM NADPH, 1 mM maleimide in 50 mM phosphate buffers (pH 5.5 to 8.8) and 30 nM enzyme, measured at 25°C.
