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. 2015 Oct 1;6:1073. doi: 10.3389/fmicb.2015.01073

Table 1.

Plasmids and primers used in this study.

Plasmids Relevant characteristic(s) Source
pET16bP pET16bP with additional multiple cloning site; allows expression of recombinant proteins with N-terminal His10-tag U. Wehmeyer
pUC57_OYERo2 pUC57 vector (2.710-kb) with additional multiple cloning site; Ampr and oyeRo2 (1.098-kb NdeI/NotI fragment) as insert Eurofins MWG operon
pEX-K4_OYERo2a pEX-K4 vector (2.507-kb) with additional multiple cloning site; Kmr and oyeRo2a (1.098-kb NdeI/NotI fragment) as insert Eurofins MWG operon
pSRoOYE2_P01 oyeRo2 of Rhodococcus opacus 1CP (1,098-kb NdeI/NotI fragment) cloned into pET16bP This study
pSRoOYE2a_P01 oyeRo2a of R. opacus 1CP (1.098-kb NdeI/NotI fragment) cloned into pET16bP This study

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