Table 1.
Plasmids | Relevant characteristic(s) | Source |
---|---|---|
pET16bP | pET16bP with additional multiple cloning site; allows expression of recombinant proteins with N-terminal His10-tag | U. Wehmeyer∗ |
pUC57_OYERo2 | pUC57 vector (2.710-kb) with additional multiple cloning site; Ampr and oyeRo2 (1.098-kb NdeI/NotI fragment) as insert | Eurofins MWG operon |
pEX-K4_OYERo2a | pEX-K4 vector (2.507-kb) with additional multiple cloning site; Kmr and oyeRo2a (1.098-kb NdeI/NotI fragment) as insert | Eurofins MWG operon |
pSRoOYE2_P01 | oyeRo2 of Rhodococcus opacus 1CP (1,098-kb NdeI/NotI fragment) cloned into pET16bP | This study |
pSRoOYE2a_P01 | oyeRo2a of R. opacus 1CP (1.098-kb NdeI/NotI fragment) cloned into pET16bP | This study |
∗Personal communication.