Table 2.
Kinetic parameters of OYERo2 proteins toward nicotinamide cofactors and maleimides.
| Proteins | Substrates | Vmax (U mg-1) | Km (μM) | kcat (s-1) | kcat/Km (μM-1 s-1) |
|---|---|---|---|---|---|
| OYERo2 | NADPHa | 49.6 | 15.4 | 34.8 | 2.3 |
| NADHa | 1.5 | 354 | 1.0 | 0.003 | |
| BNAHb | 45.2 | 380 | 31.7 | 0.08 | |
| Maleimidec | 46.4 | 3.0 | 32.5 | 10.8 | |
| N-ethylmaleimide c | 50.2 | 3.1 | 35.2 | 11.4 | |
| OYERo2a | NADPHa | 51.5 | 16.7 | 36.2 | 2.2 |
| Maleimidec | 49.6 | 4.4 | 34.9 | 7.9 |
Standard assay at 25°C with 25 mM phosphate buffer (pH 7.1), OYERo2 and OYERo2a (12.6 μg ml-1) and a1 mM maleimide or b1 mM N-ethylmaleimide or c140 μM NADPH. All values have a maximal experimental error of 10%.