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. 2015 Oct 1;6:1073. doi: 10.3389/fmicb.2015.01073

Table 2.

Kinetic parameters of OYERo2 proteins toward nicotinamide cofactors and maleimides.

Proteins Substrates Vmax (U mg-1) Km (μM) kcat (s-1) kcat/Km (μM-1 s-1)
OYERo2 NADPHa 49.6 15.4 34.8 2.3
NADHa 1.5 354 1.0 0.003
BNAHb 45.2 380 31.7 0.08
Maleimidec 46.4 3.0 32.5 10.8
N-ethylmaleimide c 50.2 3.1 35.2 11.4
OYERo2a NADPHa 51.5 16.7 36.2 2.2
Maleimidec 49.6 4.4 34.9 7.9

Standard assay at 25°C with 25 mM phosphate buffer (pH 7.1), OYERo2 and OYERo2a (12.6 μg ml-1) and a1 mM maleimide or b1 mM N-ethylmaleimide or c140 μM NADPH. All values have a maximal experimental error of 10%.