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. 2015 Oct 1;5:70. doi: 10.3389/fcimb.2015.00070

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Copyright © 2015 Lin, Cheng, Chen, Guo, Zhang, Yang, Ding, Ma, Wang and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of icmF3 affects siderophore production. Wild-type and mutant strains were grown at 37°C in CAA to stationary phase. (A) Relative levels of extracellular pyoverdine (PVD) in culture supernatants were determined spectrophotometrically. Values are presented as the mean (SD) of five independent assays. The ΔpvdA strain was used as a negative control. ^**P < 0.01. (B) Comparison of the colored halos around wild-type (top left), pchD pyochelin-negative mutant (bottom left), a pyoverdine-negative pvdA mutant (bottom right), a double pvdApchD mutant (center), and the icmF3 mutant (top right) colonies. (C) Comparison of the colored halos around PAO1 (pME6032) (bottom right), PAO-F (pME6032) (bottom left), PAO-F (pME6032-icmF3) (top) colonies. (D) Cells were cultured in CAA and levels of pchDCBA and pchEFGHI transcription in P. aeruginosa PAO1 and icmF3 mutant cells were monitored using the pchD–lacZ and pchE–lacZ transcriptional fusions, respectively. If needed, 1 mM IPTG was included in the medium for induction. The graphs show the mean and SD of two experiments performed in eight replicates each time.