(A) 3D representation of the different physical components of the rhizotron: plastic covers, polycarbonate sheets, spacers, and rubber U-channels. Blueprints are provided in Supplementary file 1. In brown, soil layer. (B) A 35-day-old plant in rhizotron with black covers removed. (C) Top view of holding box with eleven rhizotrons. (D) In vivo emission spectra of different luciferases used in this study. Transgenic homozygous lines expressing the indicated transgenes were grown on agar media for 8 days. Luciferin (300 μM) was sprayed on the seedlings and plates were kept in the dark and then imaged for 2 s at wavelengths ranging from 500 to 700 nm. Five intensity values were taken from different parts of the roots of different seedlings and averaged. Relative maximum intensity values are indicated in the lower right graph. (E) GLO1 (Growth and Luminescence Observatory 1)-imaging system. The system is composed of two back illuminated CCD cameras (a) cooled down to −55°C. A filter wheel (b) allows for spectral separation of the different luciferases. On the right, a rhizotron holder (c) is used to position the rhizotrons in front of the cameras. A stepper motor (d) rotates the rhizotron 180° to image both sides. (F) A 21 DAS plant expressing ProUBQ10:LUC2o was imaged on each of two sides of the rhizotron; luminescence signal is colorized in green or magenta to indicate side. In the middle of the panel, a combined image of the two sides is shown. The inset shows a magnified part of the root system.
DOI:
http://dx.doi.org/10.7554/eLife.07597.004
Figure 1—source data 1. Two way ANOVA p-values comparing plants grown in MS media vs plants grown in soil (pots or rhizotrons) and plants collected at day or night.We used p-value < 0.00065 threshold based on Bonferroni adjustment for multiple testing.
Figure 1—source data 2. Luminescence intensity values of the different luciferase isoforms across the emission spectrum.
Figure 1—source data 3. Gene expression values used to construct the PCA of root samples.Shoot fresh weight (FW), shoot area, lateral root number and primary root length of plants grown in different containers and media.
Figure 1—source data 4. Gene expression values used to construct the PCA of shoot samples.
Figure 1—source data 5. Shoot Fresh Weight (FW) and primary root length of plants grown with or without luciferin.
Figure 1—source data 6. Ground truth and GLO-RIA measured values of directionality, depth and width use for validation.