Table 3.
KSα amino acid sequences.
| Strain | Nearest type strain | No. of unique clones | NCBI accession no. | Top BLAST matcha (source organism) | BLAST match pathway producta (chemotypeb) | Similarity (%) |
|---|---|---|---|---|---|---|
| S12 | Kocuria gwangalliensis | 1 |
JX008015 AFO70129 |
ketosynthase (Streptomyces cyaneus) | Cur pigment (Pen) | 85.2 |
| S97* | Micromonospora aurantiaca | 1 |
KJ094408 AHN91973 |
ketosynthase (Streptomyces violaceoruber) | Granaticin (Ben) | 95.6 |
| S107* | Nocardia araoensis | 1 |
KJ094407 AHN91972 |
ketosynthase (Streptomyces sp.) | Unknown (Ang) | 95.1 |
| S78* | Nocardiopsis alba | 2 |
JX008012 AFO70126 |
ketosynthase (Streptomyces halstedii) | sch pigment (Pen) | 88.2 |
|
JX008013 AFO70127 |
ketosynthase (Streptomyces sp.) | Benastatin (Pen) | 89.2 | |||
| S92* | Nocardiopsis halotolerans | 1 |
KJ094409 AHN91974 |
ketosynthase (Streptomyces tendae) | Lysolipin (Pen) | 88.7 |
| S36* | Saccharopolyspora gloriosa | 1 |
JX008003 AFO70117 |
ketosynthase (Streptomyces antibioticus) | Simocyclinone (Ang) | 93.1 |
| S33 | Salinispora arenicola | 1 |
JX008009 AFO70123 |
ketosynthase (Streptomyces griseus) | fredericamycin (Pen) | 91.1 |
| S34 | Salinispora tropica | 1 |
JX008010 AFO70124 |
ketosynthase (Streptomyces griseus) | fredericamycin (Pen) | 90.1 |
| S10* | Streptomyces parvulus | 1 |
JX008008 AFO70122 |
ketosynthase (Streptomyces antibioticus) | simocyclinone (Ang) | 92.1 |
| S31 | Streptomyces carnosus | 1 |
JX008007 AFO70121 |
ketosynthase (Streptomyces coelicolor) | whiE pigment (Pen) | 100 |
| S39* | Streptomyces djakartensis | 1 |
JX008002 AFO70116 |
ketosynthase (Streptomyces sp.) | sch 47554 (Ang) | 93.1 |
| S40 | Streptomyces luteosporeus | 1 |
JX008004 AFO70118 |
ketosynthase (Streptomyces halstedii) | sch pigment (Pen) | 96.6 |
| S41* | Streptomyces rochei | 1 |
JX008005 AFO70119 |
ketosynthase (Streptomyces ambofaciens) | Unknown (Ang) | 99.0 |
| S71* | Streptomyces anulatus | 1 |
JX008006 AFO70120 |
ketosynthase (Streptomyces sp.) | sch 47554 (Ang) | 94.6 |
| S72* | Streptomyces xiamenensis | 1 |
JX008011 AFO70125 |
ketosynthase (Actinomadura hibisca) | Pradimicin (Ant) | 88.2 |
| S81 | Streptomyces diastaticus | 1 |
JX008014 AFO70128 |
ketosynthase (Streptomyces coelicolor) | whiE pigment (Pen) | 98.0 |
| S86 | Streptomyces griseorubens | 1 |
KJ094410 AHN91975 |
ketosynthase (Streptomyces cyaneus) | Cur pigment (Pen) | 97.0 |
a
Top BLAST matches are to the KSα domains associated with experimentally characterized biosynthetic pathways of aromatic polyketides.
b
Pen-Pentangular polyphenols, Ben-Benzoisochromanequinones, Ang-Angucyclines, Ant-Anthracyclines.
The 10 strains marked with *were selected for small-scale fermentation.