Table 2. Effect of pH on Aflatoxin B1 Detoxification by C. citriodora leaf and branch extracts.
CONTROL | PH | 3 hr |
6 hr |
12 hr |
24 hr |
48 hr |
72 hr |
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---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Toxin recovery(μg L−1) | D % | Toxin recover(μg L−1) | D % | Toxin recover(μg L−1) | D % | Toxin recover(μg L−1) | D % | Toxin recover(μg L−1) | D % | Toxin recovery(μg L−1) | D % | ||
Toxin AFB1 | 99.19r | 0.81 | 99.12r | 0.88 | 98.20r | 1.80 | 97.70r | 2.30 | 96.93qr | 3.07 | 96.19o–r | 3.81 | |
Toxin + H2O | PH 2 | 96.82pqr | 3.18 | 95.38n–r | 4.62 | 92.19k–p | 7.81 | 90.42g–l | 9.58 | 89.76e–l | 10.24 | 87.37d–j | 12.63 |
Toxin + H2O | PH 4 | 96.16o–r | 3.84 | 95.27n–r | 4.73 | 90.75h–m | 9.25 | 88.44d–l | 11.56 | 87.00d–i | 13.00 | 86.12c–h | 13.88 |
Toxin + H2O | PH 6 | 94.94m–r | 5.06 | 92.52l–q | 7.48 | 90.20f–l | 9.80 | 88.00d–l | 12.00 | 86.01c–g | 13.99 | 85.57b–f | 14.43 |
Toxin + H2O | PH 8 | 91.85j–o | 8.15 | 89.87e–l | 10.13 | 87.66d–k | 12.34 | 85.31b–e | 14.69 | 84.12a–d | 15.90 | 84.10a–d | 15.88 |
Toxin + H2O | PH 10 | 91.44i–n | 8.56 | 87.78d–k | 12.22 | 85.26b–e | 14.74 | 82.34abc | 17.66 | 81.19ab | 18.81 | 80.06a | 19.94 |
Toxin + H2O | WpH | 98.90r | 1.10 | 97.36r | 2.64 | 96.77pqr | 3.23 | 96.44o–r | 3.56 | 96.03o–r | 3.97 | 95.81n-r | 4.19 |
TREATMENT | |||||||||||||
Corymbia Leaf + AFB1 Corymbia | PH 2 | 38.92h–u | 61.08 | 34.16c–u | 65.84 | 29.40a–t | 70.60 | 24.63a–o | 75.37 | 19.87a–l | 80.13 | 15.10a–j | 84.90 |
PH 4 | 31.67b–u | 68.33 | 27.45a–r | 72.55 | 23.24a–n | 76.76 | 19.02a–l | 80.98 | 14.81a–j | 85.19 | 10.59a–i | 89.41 | |
PH 6 | 26.53a–q | 73.47 | 22.66a–n | 77.34 | 18.79a–l | 81.21 | 14.92a–j | 85.08 | 11.05a–i | 88.95 | 7.17a–e | 92.83 | |
PH 8 | 22.95a–n | 77.05 | 18.36a–l | 81.64 | 13.77a–j | 86.23 | 9.17a–g | 90.83 | 7.70a–f | 92.30 | 4.79abc | 95.21 | |
PH 10 | 15.49a–j | 84.51 | 12.47a–j | 87.53 | 9.46a–h | 90.54 | 6.44a–d | 93.56 | 3.43ab | 96.57 | 0.41a | 99.59 | |
WpH | 39.59i–u | 60.41 | 35.16d–u | 64.84 | 30.74b-t | 69.26 | 26.31a–q | 73.69 | 21.88a–m | 78.12 | 17.45a–k | 82.55 | |
Branch + AFB1 | PH 2 | 57.42stu | 42.58 | 56.26r–u | 43.74 | 53.97o–u | 46.03 | 50.27m–u | 49.73 | 41.93j–u | 58.07 | 39.94i–u | 60.06 |
PH 4 | 54.79p–u | 45.21 | 53.32o–u | 46.68 | 51.50n–u | 48.50 | 49.75m–u | 50.25 | 46.18k–u | 53.82 | 38.14g–u | 61.86 | |
PH 6 | 41.68j–u | 58.32 | 37.54g–u | 62.46 | 34.21c–u | 65.79 | 31.50b–u | 68.50 | 25.54a–q | 74.46 | 22.92a–n | 77.08 | |
PH 8 | 36.65e–u | 63.35 | 35.46d–u | 64.54 | 32.00b–u | 68.00 | 29.91a–t | 70.09 | 25.31a–p | 74.69 | 21.71a–m | 78.29 | |
PH 10 | 34.74d–u | 65.26 | 31.46b–u | 68.54 | 28.60a–t | 71.40 | 28.01a–s | 71.99 | 21.75a–m | 78.25 | 16.04a–j | 83.96 | |
WpH | 61.07u | 38.93 | 58.11tu | 41.89 | 47.81q–u | 45.01 | 47.81l–u | 52.19 | 36.94f–u | 63.06 | 30.87b–t | 69.13 |
Data were analyzed by analysis of Variance (ANOVA). Values with different alphabetic letters indicate significant differences (P < 0.05) as determined by Tukey’s Multiple Range test. D%: Detoxification %age. WpH: without pH.