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. 2015 Oct 1;5:14672. doi: 10.1038/srep14672

Table 5. Percent mortality of brine shrimps (Artemia salina) larvae at 26 °C after treatment with toxin (AFB1 & AFB2) detoxified with E. citriodora leaves extract at various incubation periods.

TREATMENTS Incubation period (hrs.) No. of living shrimps No. of dead shrimps % Mortality
CONTROL
Sea water + shrimps 24 40 0 0
48 40 0 0
72 40 0 0
96 39 1 2.5
Methanol + shrimps 24 38 1 2.5
48 38 2 5
72 37 3 7.5
96 36 3 7.5
Untreated toxins + shrimps 24 7 33 82.5
48 5 35 87.5
72 4 36 90
96 3 37 92.5
TREATMENT
Treated toxin with E. citriodora leaves extract + shrimps 24 35 5 12.5
48 34 6 15
72 34 6 15
96 33 7 17.5

Sea water was taken as a control. Other Controls consist of Methanol and untreated toxin AFB1 (100 μg L−1) and AFB2 (50 μg L−1) dried and redissolved in sea water. Values are mean of three replicates. Data were analyzed by analysis of Variance (ANOVA).