Table 5. Percent mortality of brine shrimps (Artemia salina) larvae at 26 °C after treatment with toxin (AFB1 & AFB2) detoxified with E. citriodora leaves extract at various incubation periods.
TREATMENTS | Incubation period (hrs.) | No. of living shrimps | No. of dead shrimps | % Mortality |
---|---|---|---|---|
CONTROL | ||||
Sea water + shrimps | 24 | 40 | 0 | 0 |
48 | 40 | 0 | 0 | |
72 | 40 | 0 | 0 | |
96 | 39 | 1 | 2.5 | |
Methanol + shrimps | 24 | 38 | 1 | 2.5 |
48 | 38 | 2 | 5 | |
72 | 37 | 3 | 7.5 | |
96 | 36 | 3 | 7.5 | |
Untreated toxins + shrimps | 24 | 7 | 33 | 82.5 |
48 | 5 | 35 | 87.5 | |
72 | 4 | 36 | 90 | |
96 | 3 | 37 | 92.5 | |
TREATMENT | ||||
Treated toxin with E. citriodora leaves extract + shrimps | 24 | 35 | 5 | 12.5 |
48 | 34 | 6 | 15 | |
72 | 34 | 6 | 15 | |
96 | 33 | 7 | 17.5 |
Sea water was taken as a control. Other Controls consist of Methanol and untreated toxin AFB1 (100 μg L−1) and AFB2 (50 μg L−1) dried and redissolved in sea water. Values are mean of three replicates. Data were analyzed by analysis of Variance (ANOVA).