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. 2015 Oct 1;5:14766. doi: 10.1038/srep14766

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(a) Image intensity decay caused by depletion alone in Sample #1. Red curves are fitting to mono-exponential decay. Black curves represent background photobleaching caused by a low power (16 μW) excitation laser beam, which do not decay. (b) Comparison of STED survival time , excitation-only survival time , depletion-only time constant , and (would-be survival time if two lasers caused photobleaching independently). One can see that .

Inline graphic — (a) Image intensity decay caused by depletion alone in Sample #1. Red curves are fitting to mono-exponential decay. Black curves represent background photobleaching caused by a low power (16 μW) excitation laser beam, which do not decay. (b) Comparison of STED survival time , excitation-only survival time , depletion-only time constant , and (would-be survival time if two lasers caused photobleaching independently). One can see that .