Figure 5.

Effects of HDAC6 knock-out on tubulin acetylation and brain-derived neurotrophic factor (BDNF) protein levels. Representative western blots showing acetylated alpha-tubulin and mature BDNF in single (HDAC6-WT+R6/1, HDAC6-KO+HD-ntg), double mutant (HDAC6-KO+R6/1), and wt (HDAC6-WT+HD-ntg) mice for HDAC6 and HD mutations for both striatum and cortex (A). Acetylated tubulin was normalized to tubulin level and the ratios (in %) of acetylated tubulin/total amount of tubulin were shown for striatum (B) and cortex (C). The same normalization was performed for mature BDNF and relative BDNF levels were shown for striatum (D) and cortex (E) in single, double mutant, and wt mice. *P < 0.05, **P < 0.01 by Mann–Whitney test.