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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Stem Cells. 2015 Jul 14;33(10):2925–2935. doi: 10.1002/stem.2084

Figure 2.

Figure 2

miR-302 directly regulates BAF170 and BAF53a in hESCs. (A) Inhibition of the miR-302 family in H1 hESCs. Sequestration of mature miR-302 is evident by inability to amplify miR-302a and miR-302c by qRT-PCR. (B) mRNA levels of Brg1 subunits measured by qRT-PCR following inhibition of miR-302 in hESCs compared to negative control. (C) Protein level of Brg1 complex subunits upon miR-302 inhibition in hESCs compared to negative control. (D) Luciferase activity of BAF170 WT and mutant 3′UTR reporter constructs cotransfected into HeLa cells with pre-miR-302a or negative control precursor. (E) Luciferase activity of BAF53a WT and mutant 3′UTR reporter constructs cotransfected into HeLa cells with pre-miR-302a or negative control precursor. In all panels, error bars represent standard deviation of at least three independent replicates. * p < 0.001, ** p < 0.01, *** p < 0.05