Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Stem Cells. 2015 Jul 14;33(10):2925–2935. doi: 10.1002/stem.2084

Figure 3.

Figure 3

Differentiation-induced upregulation of BAF170 and BAF53a mirrors miR-302 downregulation. (A) mRNA levels of miR302a, Oct4 and Brg1 complex subunits measured by qRT-PCR in H1 hESCs treated with 1 μM retinoic acid for the indicated time periods. (B) Protein levels of Brg1 complex subunits and Oct4 during RA-induced differentiation. (C) An assessment of the effects of RA-induced differentiation on H1 ESCs, using pluripotency markers and lineage markers for each of the three germ layers. mRNA levels of the indicated pluripotency genes and lineage markers were measured by qRT-PCR in H1 hESCs treated with 1 μM retinoic acid for the indicated time periods.