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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Stem Cells. 2015 Jul 14;33(10):2925–2935. doi: 10.1002/stem.2084

Figure 4.

Figure 4

BAF170 overexpression and miR-302 inhibition both affect cell cycle and proliferation genes. (A) Western blot of BAF170 levels in cells lines stably transduced with empty lentiviral vector (EV) or lentiviral vector containing the BAF170 ORF (OE). Actin was used as a loading control. (B) Phase contrast microscopy of EV and OE cell lines three days after passaging showing characteristic hESC morphology. (C) mRNA levels of BAF170, OCT4, SOX2, and NANOG in the EV and OE cell lines. (D) Venn diagram demonstrating the overlap in gene sets misregulated upon miR-302 inhibition or BAF170 overexpression based on microarray gene expression analysis. (E) Top ten functional categories enriched in the set of genes misregulated in the BAF170 OE cell line. (F) Top ten functional categories enriched in the set of miR-302-regulated genes.