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CenH3 is located in distinct foci in the nucleus. Strain Zt121-85 was grown on YMS medium for 24 h at 18 °C; images from a representative spore are shown. CenH3 is localized inside the nucleus and organized in several spots (between 4 and 7 foci can be distinguished by epifluorescence microscopy); there were no spores with single chromocenters. Left, Fluorescence microscopy of GFP–CenH3. Center, Bright field image. Right, Merged images. Scale bar 5 μm
