Figure 1 .

Characterization of K-CAR in various donors. (A) RT-PCR was employed to detect the expression of 6H5 scFv (700 bp) using scFv specific primers. Amplified β-actin was used as a loading control, and scFv plasmid was used as a positive control. Expression of 6H5 scFv was demonstrated in K-CAR T cells obtained from BC patients and NDs. No scFv expression was detected in control T cells or PBMCs. (B) Both Fc⁺ and CD3⁺ T cell populations were determined in T cells transfected with K-CAR or GFP from patient 157 (top panel) and ND1 (bottom panel) by FACS using anti-Fc and CD3 antibodies on days 7, 21 and 35 post-transfection. The isotype alone was used as control. (C) Both FOXP3⁺ and CD8⁺ or CD4⁺ T cells from BC patient 243 were determined by FACS (left panel). The percentage of CD8⁺ T cells was increased in K-CAR T cells after CD4⁺ depletion (right panel). (D) Lower percentages of CD8⁺ and higher percentages of CD4⁺ T cells were demonstrated in K-CAR T cells obtained from BC patients than from NDs (top panel). Significantly enhanced CD8⁺ (P = 0.0003) and reduced CD4⁺ (P = 0.0004) T cell populations were demonstrated in T cells from BC patients (n = 7) after CD4 depletion.

Figure 1.

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Figure 1.

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Figure 1.

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