Table 1.

Association constants (Ka) for Gp6 and mycobacterial surface oligosaccharides measured at 25°C and pH 7.2 using the direct ESI-MS assay.¹

#	Bacterial oligosaccharide structures	MW, Da	Ka, M-1 × 103
1	α-Manp-(1→5)-β-Araf-(1→2)-α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	931	3.9 ± 1.1
2	α-Manp-(1→2)-α-Manp-(1→5)-β-Araf-(1→2)-α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	1094	4.4 ± 3.9
3	β-Araf-(1→2)-α-Araf-(1→5)[β-Araf-(1→2)-α-Araf-(1→3)]-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	1034	3.6 ± 0.4
4	β-Araf-(1→2)-α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	770	NB2
5	α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	638	7.6 ± 3.2
6	α-Araf-(1→5)-[α-Araf-(1→3)]-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	770	2.4 ± 0.7
7	β-Araf-(1→2)-α-Araf-(1→3)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	770	1.1 ± 0.2
8	α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	669	6.9 ± 0.8
9	α-Manp-(1→2)-α-Manp-(1→2)-α-Manp-(1→5)-β-Araf-(1→2)-α-Araf-(1→5)-α-Araf-(1→5)-α-Araf-octyl-NHCOCF3	1256	2.6 ± 1.1

¹Fucose (Fuc) and rhamnose (Rha) residues are in the L-configuration; mannose (Man), arabinose (Ara) and glucose (Glc) residues are in the D-configuration.

²NB – no binding detected.