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. 2015 Jun 4;4(10):e1031440. doi: 10.1080/2162402X.2015.1031440

Figure 4.

Figure 4.

miR-197 suppresses proliferation, invasion but promotes apoptosis in HepG2 cells through IL-6/STAT3 signaling pathway. (A–B) HepG2 cells growth were measured by CCK-8 analysis (A) or using EdU incorporation (B) after transfection of negative control (NC), miR-197 mimics (miR-197) or miR-197 plus pcDNA-STAT3 which contain STAT3 cDNA that cannot be targeted by miR-197 (miR-197+STAT3). (C) Apoptotic HepG2 cells were analyzed by FACS after they are transfected with NC, miR-197 or miR-197+STAT3. The AnnexinV-positive cells were regarded as apoptotic cells. (D) The invasive ability of HepG2 cells was evaluated by in vitro invasion assays after transfection of NC, miR-197 or miR-197+STAT3. (E) HepG2 cells were transfected with NC, miR-197 or miR-197+STAT3, 48 h later, the expression levels of STAT3, p-STAT3, c-Myc, Bcl−2, and MMP-2 were analyzed by Western blotting. Data are shown as mean ± s.d. (n = 3) of one representative experiment. Similar results were obtained in at least three independent experiments. %p < 0.05, %%p < 0.01, %%%p < 0.001. Scale bar = 50 μm.