Figure 4.

c-Fos immunohistochemistry in the cingulate cortex of the sham-operated (sham; A) and myocardial infarction (MI) rats at 3 (B), 7 (C), 14 (D), 28 (E) and 56 days (F) after MI.

In the sham group, c-Fos immunoreactivity was hardly detected in the cingulate cortex. In the MI groups, the number of c-Fos-positive (c-Fos⁺) cells in the cingulate cortex (arrows) was increased at 3 d after MI, peaked at 14 d after MI, and returned to the level of the sham group at 56 d after MI. Scale bar: 100 μm. (G) The mean number of c-Fos⁺ cells in the cingulate cortex (n = 7 rats per group); *P < 0.05, vs. sham group. One-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).