Fig. 5.

Cellulase detection using indirect immunofluorescence in the Δacy1 mutant strain. The fungus was grown in sophorose for 6 h in the presence or absence of dibutyryl-cAMP (dbcAMP). To detect cellulase in the mycelia we used a rabbit polyclonal anti-Trichoderma viride cellulose (CEL7A) antibody diluted 1:1000, and an Alexa Fluor 488-conjgated secondary antibody diluted 1:5000. Furthermore, the fluorochrome DAPI was used to detect cell nuclei. Images were acquired on a Leica TCS SP5 confocal microscope. Lens 40x, and zoom 3.8