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Inhibitory effects of 2-HT, kojic acid and arbutin on α-MSH-induced melanin production in B16 melanoma cells. The pellets were resuspended in 2 mol/L NaOH solution and absorbance of supernatants was measured at 450 nm using a microplate reader. (●) 2-HT, (■) kojic acid and (▲) arbutin.
